Directed Growth o f Fungal Hyphae in an Electric Field A Biophysical Analysis
نویسندگان
چکیده
Galvanotropism, Dose-Response Curve, Mycelial Growth The galvanotropic response of the mycelial fungus Neurospora crassa is investigated. The angle distribution function of growing hyphae is described by a generating function which contains two non-trivial terms; one for directional growth and one for bidirectional growth. The following results were obtained, (i) Germ tubes grew towards the anode, (ii) The cellular response was linear for small sized cells and weaker electric fields. The galvanotropic constant, Kgrow, which describes the linear response, was small for short germ tubes or hyphae (A^grow 1 = _ 20 V-cm“1 for /0 = 10 |am) and large for longer cells (A^grow1 = _ 1 -7 V cm "1 for 10 = 100 |im). The growth coefficient K P o f (-18 .5 mV) describes the response independent of cell size. The linear response is explained by the field-induced distribution of charged mem brane-bound proteins essential for galvanotropism. (iii) For E > E0 3.8 V -cm “1, the linear response is inhibited (inhibition coefficient K x = 1.11). The inhibition is explained by field-in duced changes of the membrane potential, (iv) The galvanotropic response o f longer hyphae was bidirectional. The cells grew on average perpendicular to the applied field. The bidirec tional response is proportional to Er with a bidirectional growth coefficient K2 o f -(0.25 V)“2. The bidirectional growth is explained by the inhibition of the directed growth process. The transition from anode-directed to bidirectional growth was a function of the applied electric field as well as of the tube length (directed growth for E2-l0< 4 A V2-cm“ ' and bidirectional growth for E?-l{)> 4.4 V2• cm“1).
منابع مشابه
Comparison of six DNA extraction methods for recovery of fungal DNA as assessed by quantitative PCR.
The detection of fungal pathogens in clinical samples by PCR requires the use of extraction methods that efficiently lyse fungal cells and recover DNA suitable for amplification. We used quantitative PCR assays to measure the recovery of DNA from two important fungal pathogens subjected to six DNA extraction methods. Aspergillus fumigatus conidia or Candida albicans yeast cells were added to br...
متن کاملهمراهی کیست هیداتید ریه با عفونت قارچی: گزارش موردی
Background: Hydatid cyst is a zoonosis rarely occurred except in endemic areas that capable of making pulmonary cavities fascilating fungus growth within it. Aspergillo-ma is a glob formed by hyphae from saprophyte growth of aspergillous specious in previously performed cavities within pulmonary parenchyma. Case report: A 28 years old male patient without any comorbidity presented in emergency...
متن کاملRelationship between fluorescein diacetate-stained hyphae and oxygen utilization, glucose utilization, and biomass of submerged fungal batch cultures.
The relationship between fungal activity and staining with fluorescein diacetate (FDA) was investigated by growing Penicillium citrinum and Rhizoctonia solani in submerged batch cultures at different initial glucose concentrations and aeration rates. A modified FDA staining method, similar to the Jones and Mollison technique (P. Jones and J. Mollison, J. Gen. Microbiol. 2:54-69, 1948), was deve...
متن کاملMapping the growth of fungal hyphae: orthogonal cell wall expansion during tip growth and the role of turgor.
By computer-enhanced videomicroscopy, we mapped the trajectory of external and internal cell surface markers in growing fungal hyphae to determine the pattern of cell wall expansion during apical growth. Carbon particles (India ink) were chosen as external markers for tip expansion of Rhizoctonia solani hyphae. Irregularities in the growing apical walls of R. solani served as internal markers. ...
متن کاملArbuscular Mycorrhizal Fungi Promote the Growth of Ceratocarpus arenarius (Chenopodiaceae) with No Enhancement of Phosphorus Nutrition
The mycorrhizal status of plants in the Chenopodiaceae is not well studied with a few controversial reports. This study examined arbuscular mycorrhizal (AM) colonization and growth response of Ceratocarpus arenarius in the field and a greenhouse inoculation trial. The colonization rate of AM fungi in C. arenarius in in-growth field cores was low (around 15%). Vesicles and intraradical hyphae we...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2013